resource source identifier toxin b tcdb protein (MedChemExpress)
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resource source identifier toxin b tcdb protein
Resource Source Identifier Toxin B Tcdb Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/resource+source+identifier+toxin+b+tcdb+protein/Toxin+B%2FTcdB%2C+C%2E+difficile/pm40716062-752-2-11
Average 93 stars, based on 1 article reviews
Resource Source Identifier Toxin B Tcdb Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/resource+source+identifier+toxin+b+tcdb+protein/Toxin+B%2FTcdB%2C+C%2E+difficile/pm40716062-752-2-11
Average 93 stars, based on 1 article reviews
resource source identifier toxin b tcdb protein - by Bioz Stars,
2026-09
93/100 stars
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Recombinant:Article Title: The Hippo pathway kinase MST1 mediates a feedback loop to maintain NLRP3 inflammasome homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Toxin B/TcdB protein, C. difficile (His) MedChemExpress Cat# HY-P79219 Poly(dA:dT)/LyoVecTM Invivogen Cat# tlrl-patc Caspase-1 (human), (recombinant) (active) Enzo Cat# ALX-201-056 MSU Invivogen Cat# tlrl-msu M-CSF Thermo Scientific Cat# PMC2044 DSS Thermo Scientific Cat# 21655 Staurosporine Solarbio Cat# S5840 ATP gamma S Kinase substrate Abcam Cat# ab138911 p-Nitrobenzyl mesylate, Alkylation reagent Abcam Cat# ab138910 Propidium Iodide MedChemExpress Cat# HY-D0815 Polybrene Sigma Cat# H9268 Digitonin Sigma Cat# D141 Doxycycline hydrochloride MedChemExpress Cat# HY-N0565A LipofectamineTM 3000 Reagent Invitrogen Cat# L3000015 Critical commercial assays Hematoxylin and Eosin Staining Kit Beyotime Cat# C0105 Human IL-6 Uncoated ELISA Kit Invitrogen Cat# 88-7066-22 Human TNF alpha Uncoated ELISA Kit Invitrogen Cat# 88-7346-22 Mouse TNF alpha Uncoated ELISA Kit Invitrogen Cat# 88-7324-22 Mouse IL-1 beta Uncoated ELISA Kit Invitrogen Cat# 88-7013A-22 Human Interleukin 1 Beta (IL-1β) ELISA Kit Shanghai Jianglai Biotechnology Cat# JL13662 LDH Cytotoxicity Assay Kit Beyotime Cat# C0017 RNAsimple Total RNA Kit TIANGEN Cat# DP419 Lentivirus Packaging Vectors Set A Beyotime Cat# L00002S 2X Universal SYBR Green Fast qPCR Mix ABclonal Cat# RK21203 ABScript III RT Master Mix ABclonal Cat# RK20429 Plasmid Midi Kit Qiagen Cat# 12143 Anti-HA Affinity Gel GNI Cat# GNI4510-HA-M Anti-Flag Affinity Gel GNI Cat# GNI4510-FG-M Protein G Plus/Protein A-Aga Merck Millipore Cat# IP10-10ML PierceTM Glutathione Agarose ThermoFisher Cat# 16100 HisPur TM Ni-NTA Resin ThermoFisher Cat# 88221 2X MultiF Seamless Assembly Mix ABclonal Cat# RK21020 Experimental models: Cell lines Human HEK293T cells ATCC ATCC CRL-11268; RRID: CVCL_1926 Human THP-1 cells ATCC ATCC TIB-202; RRID: CVCL_0006 Human HeLa cells ATCC ATCC: CCL-2; RRID: CVCL_0030 Experimental models: Organisms/strains C57BL/6J mice Jackson Laboratory Cat# 000664 Mst1− /− mice Provided by Dr. Dawang Zhou Previously described in (Zhou et al., 2008) Oligonucleotides qRT-PCR primer for NLRP3 forward: 5′- CTTCTCTGATGAGGCCCAAG-3′ This paper N/A qRT-PCR primer for NLRP3 reverse: 5′- GCAGCAAACTGGAAAGGAAG-3′ This paper N/A qRT-PCR primer for IL-1β forward: 5′- GGGCCTCAAGGAAAAGAATC-3′ This paper N/A qRT-PCR primer for IL-1β reverse: 5′- TTCTGCTTGAGAGGTGCTGA-3′ This paper N/A (Continued on next page) Cell Reports 44, 116076, August 26, 2025 19 .. REAGENT or Staining:Article Title: The Hippo pathway kinase MST1 mediates a feedback loop to maintain NLRP3 inflammasome homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Toxin B/TcdB protein, C. difficile (His) MedChemExpress Cat# HY-P79219 Poly(dA:dT)/LyoVecTM Invivogen Cat# tlrl-patc Caspase-1 (human), (recombinant) (active) Enzo Cat# ALX-201-056 MSU Invivogen Cat# tlrl-msu M-CSF Thermo Scientific Cat# PMC2044 DSS Thermo Scientific Cat# 21655 Staurosporine Solarbio Cat# S5840 ATP gamma S Kinase substrate Abcam Cat# ab138911 p-Nitrobenzyl mesylate, Alkylation reagent Abcam Cat# ab138910 Propidium Iodide MedChemExpress Cat# HY-D0815 Polybrene Sigma Cat# H9268 Digitonin Sigma Cat# D141 Doxycycline hydrochloride MedChemExpress Cat# HY-N0565A LipofectamineTM 3000 Reagent Invitrogen Cat# L3000015 Critical commercial assays Hematoxylin and Eosin Staining Kit Beyotime Cat# C0105 Human IL-6 Uncoated ELISA Kit Invitrogen Cat# 88-7066-22 Human TNF alpha Uncoated ELISA Kit Invitrogen Cat# 88-7346-22 Mouse TNF alpha Uncoated ELISA Kit Invitrogen Cat# 88-7324-22 Mouse IL-1 beta Uncoated ELISA Kit Invitrogen Cat# 88-7013A-22 Human Interleukin 1 Beta (IL-1β) ELISA Kit Shanghai Jianglai Biotechnology Cat# JL13662 LDH Cytotoxicity Assay Kit Beyotime Cat# C0017 RNAsimple Total RNA Kit TIANGEN Cat# DP419 Lentivirus Packaging Vectors Set A Beyotime Cat# L00002S 2X Universal SYBR Green Fast qPCR Mix ABclonal Cat# RK21203 ABScript III RT Master Mix ABclonal Cat# RK20429 Plasmid Midi Kit Qiagen Cat# 12143 Anti-HA Affinity Gel GNI Cat# GNI4510-HA-M Anti-Flag Affinity Gel GNI Cat# GNI4510-FG-M Protein G Plus/Protein A-Aga Merck Millipore Cat# IP10-10ML PierceTM Glutathione Agarose ThermoFisher Cat# 16100 HisPur TM Ni-NTA Resin ThermoFisher Cat# 88221 2X MultiF Seamless Assembly Mix ABclonal Cat# RK21020 Experimental models: Cell lines Human HEK293T cells ATCC ATCC CRL-11268; RRID: CVCL_1926 Human THP-1 cells ATCC ATCC TIB-202; RRID: CVCL_0006 Human HeLa cells ATCC ATCC: CCL-2; RRID: CVCL_0030 Experimental models: Organisms/strains C57BL/6J mice Jackson Laboratory Cat# 000664 Mst1− /− mice Provided by Dr. Dawang Zhou Previously described in (Zhou et al., 2008) Oligonucleotides qRT-PCR primer for NLRP3 forward: 5′- CTTCTCTGATGAGGCCCAAG-3′ This paper N/A qRT-PCR primer for NLRP3 reverse: 5′- GCAGCAAACTGGAAAGGAAG-3′ This paper N/A qRT-PCR primer for IL-1β forward: 5′- GGGCCTCAAGGAAAAGAATC-3′ This paper N/A qRT-PCR primer for IL-1β reverse: 5′- TTCTGCTTGAGAGGTGCTGA-3′ This paper N/A (Continued on next page) Cell Reports 44, 116076, August 26, 2025 19 .. REAGENT or Enzyme-linked Immunosorbent Assay:Article Title: The Hippo pathway kinase MST1 mediates a feedback loop to maintain NLRP3 inflammasome homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Toxin B/TcdB protein, C. difficile (His) MedChemExpress Cat# HY-P79219 Poly(dA:dT)/LyoVecTM Invivogen Cat# tlrl-patc Caspase-1 (human), (recombinant) (active) Enzo Cat# ALX-201-056 MSU Invivogen Cat# tlrl-msu M-CSF Thermo Scientific Cat# PMC2044 DSS Thermo Scientific Cat# 21655 Staurosporine Solarbio Cat# S5840 ATP gamma S Kinase substrate Abcam Cat# ab138911 p-Nitrobenzyl mesylate, Alkylation reagent Abcam Cat# ab138910 Propidium Iodide MedChemExpress Cat# HY-D0815 Polybrene Sigma Cat# H9268 Digitonin Sigma Cat# D141 Doxycycline hydrochloride MedChemExpress Cat# HY-N0565A LipofectamineTM 3000 Reagent Invitrogen Cat# L3000015 Critical commercial assays Hematoxylin and Eosin Staining Kit Beyotime Cat# C0105 Human IL-6 Uncoated ELISA Kit Invitrogen Cat# 88-7066-22 Human TNF alpha Uncoated ELISA Kit Invitrogen Cat# 88-7346-22 Mouse TNF alpha Uncoated ELISA Kit Invitrogen Cat# 88-7324-22 Mouse IL-1 beta Uncoated ELISA Kit Invitrogen Cat# 88-7013A-22 Human Interleukin 1 Beta (IL-1β) ELISA Kit Shanghai Jianglai Biotechnology Cat# JL13662 LDH Cytotoxicity Assay Kit Beyotime Cat# C0017 RNAsimple Total RNA Kit TIANGEN Cat# DP419 Lentivirus Packaging Vectors Set A Beyotime Cat# L00002S 2X Universal SYBR Green Fast qPCR Mix ABclonal Cat# RK21203 ABScript III RT Master Mix ABclonal Cat# RK20429 Plasmid Midi Kit Qiagen Cat# 12143 Anti-HA Affinity Gel GNI Cat# GNI4510-HA-M Anti-Flag Affinity Gel GNI Cat# GNI4510-FG-M Protein G Plus/Protein A-Aga Merck Millipore Cat# IP10-10ML PierceTM Glutathione Agarose ThermoFisher Cat# 16100 HisPur TM Ni-NTA Resin ThermoFisher Cat# 88221 2X MultiF Seamless Assembly Mix ABclonal Cat# RK21020 Experimental models: Cell lines Human HEK293T cells ATCC ATCC CRL-11268; RRID: CVCL_1926 Human THP-1 cells ATCC ATCC TIB-202; RRID: CVCL_0006 Human HeLa cells ATCC ATCC: CCL-2; RRID: CVCL_0030 Experimental models: Organisms/strains C57BL/6J mice Jackson Laboratory Cat# 000664 Mst1− /− mice Provided by Dr. Dawang Zhou Previously described in (Zhou et al., 2008) Oligonucleotides qRT-PCR primer for NLRP3 forward: 5′- CTTCTCTGATGAGGCCCAAG-3′ This paper N/A qRT-PCR primer for NLRP3 reverse: 5′- GCAGCAAACTGGAAAGGAAG-3′ This paper N/A qRT-PCR primer for IL-1β forward: 5′- GGGCCTCAAGGAAAAGAATC-3′ This paper N/A qRT-PCR primer for IL-1β reverse: 5′- TTCTGCTTGAGAGGTGCTGA-3′ This paper N/A (Continued on next page) Cell Reports 44, 116076, August 26, 2025 19 .. REAGENT or LDH Cytotoxicity Assay:Article Title: The Hippo pathway kinase MST1 mediates a feedback loop to maintain NLRP3 inflammasome homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Toxin B/TcdB protein, C. difficile (His) MedChemExpress Cat# HY-P79219 Poly(dA:dT)/LyoVecTM Invivogen Cat# tlrl-patc Caspase-1 (human), (recombinant) (active) Enzo Cat# ALX-201-056 MSU Invivogen Cat# tlrl-msu M-CSF Thermo Scientific Cat# PMC2044 DSS Thermo Scientific Cat# 21655 Staurosporine Solarbio Cat# S5840 ATP gamma S Kinase substrate Abcam Cat# ab138911 p-Nitrobenzyl mesylate, Alkylation reagent Abcam Cat# ab138910 Propidium Iodide MedChemExpress Cat# HY-D0815 Polybrene Sigma Cat# H9268 Digitonin Sigma Cat# D141 Doxycycline hydrochloride MedChemExpress Cat# HY-N0565A LipofectamineTM 3000 Reagent Invitrogen Cat# L3000015 Critical commercial assays Hematoxylin and Eosin Staining Kit Beyotime Cat# C0105 Human IL-6 Uncoated ELISA Kit Invitrogen Cat# 88-7066-22 Human TNF alpha Uncoated ELISA Kit Invitrogen Cat# 88-7346-22 Mouse TNF alpha Uncoated ELISA Kit Invitrogen Cat# 88-7324-22 Mouse IL-1 beta Uncoated ELISA Kit Invitrogen Cat# 88-7013A-22 Human Interleukin 1 Beta (IL-1β) ELISA Kit Shanghai Jianglai Biotechnology Cat# JL13662 LDH Cytotoxicity Assay Kit Beyotime Cat# C0017 RNAsimple Total RNA Kit TIANGEN Cat# DP419 Lentivirus Packaging Vectors Set A Beyotime Cat# L00002S 2X Universal SYBR Green Fast qPCR Mix ABclonal Cat# RK21203 ABScript III RT Master Mix ABclonal Cat# RK20429 Plasmid Midi Kit Qiagen Cat# 12143 Anti-HA Affinity Gel GNI Cat# GNI4510-HA-M Anti-Flag Affinity Gel GNI Cat# GNI4510-FG-M Protein G Plus/Protein A-Aga Merck Millipore Cat# IP10-10ML PierceTM Glutathione Agarose ThermoFisher Cat# 16100 HisPur TM Ni-NTA Resin ThermoFisher Cat# 88221 2X MultiF Seamless Assembly Mix ABclonal Cat# RK21020 Experimental models: Cell lines Human HEK293T cells ATCC ATCC CRL-11268; RRID: CVCL_1926 Human THP-1 cells ATCC ATCC TIB-202; RRID: CVCL_0006 Human HeLa cells ATCC ATCC: CCL-2; RRID: CVCL_0030 Experimental models: Organisms/strains C57BL/6J mice Jackson Laboratory Cat# 000664 Mst1− /− mice Provided by Dr. Dawang Zhou Previously described in (Zhou et al., 2008) Oligonucleotides qRT-PCR primer for NLRP3 forward: 5′- CTTCTCTGATGAGGCCCAAG-3′ This paper N/A qRT-PCR primer for NLRP3 reverse: 5′- GCAGCAAACTGGAAAGGAAG-3′ This paper N/A qRT-PCR primer for IL-1β forward: 5′- GGGCCTCAAGGAAAAGAATC-3′ This paper N/A qRT-PCR primer for IL-1β reverse: 5′- TTCTGCTTGAGAGGTGCTGA-3′ This paper N/A (Continued on next page) Cell Reports 44, 116076, August 26, 2025 19 .. REAGENT or SYBR Green Assay:Article Title: The Hippo pathway kinase MST1 mediates a feedback loop to maintain NLRP3 inflammasome homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Toxin B/TcdB protein, C. difficile (His) MedChemExpress Cat# HY-P79219 Poly(dA:dT)/LyoVecTM Invivogen Cat# tlrl-patc Caspase-1 (human), (recombinant) (active) Enzo Cat# ALX-201-056 MSU Invivogen Cat# tlrl-msu M-CSF Thermo Scientific Cat# PMC2044 DSS Thermo Scientific Cat# 21655 Staurosporine Solarbio Cat# S5840 ATP gamma S Kinase substrate Abcam Cat# ab138911 p-Nitrobenzyl mesylate, Alkylation reagent Abcam Cat# ab138910 Propidium Iodide MedChemExpress Cat# HY-D0815 Polybrene Sigma Cat# H9268 Digitonin Sigma Cat# D141 Doxycycline hydrochloride MedChemExpress Cat# HY-N0565A LipofectamineTM 3000 Reagent Invitrogen Cat# L3000015 Critical commercial assays Hematoxylin and Eosin Staining Kit Beyotime Cat# C0105 Human IL-6 Uncoated ELISA Kit Invitrogen Cat# 88-7066-22 Human TNF alpha Uncoated ELISA Kit Invitrogen Cat# 88-7346-22 Mouse TNF alpha Uncoated ELISA Kit Invitrogen Cat# 88-7324-22 Mouse IL-1 beta Uncoated ELISA Kit Invitrogen Cat# 88-7013A-22 Human Interleukin 1 Beta (IL-1β) ELISA Kit Shanghai Jianglai Biotechnology Cat# JL13662 LDH Cytotoxicity Assay Kit Beyotime Cat# C0017 RNAsimple Total RNA Kit TIANGEN Cat# DP419 Lentivirus Packaging Vectors Set A Beyotime Cat# L00002S 2X Universal SYBR Green Fast qPCR Mix ABclonal Cat# RK21203 ABScript III RT Master Mix ABclonal Cat# RK20429 Plasmid Midi Kit Qiagen Cat# 12143 Anti-HA Affinity Gel GNI Cat# GNI4510-HA-M Anti-Flag Affinity Gel GNI Cat# GNI4510-FG-M Protein G Plus/Protein A-Aga Merck Millipore Cat# IP10-10ML PierceTM Glutathione Agarose ThermoFisher Cat# 16100 HisPur TM Ni-NTA Resin ThermoFisher Cat# 88221 2X MultiF Seamless Assembly Mix ABclonal Cat# RK21020 Experimental models: Cell lines Human HEK293T cells ATCC ATCC CRL-11268; RRID: CVCL_1926 Human THP-1 cells ATCC ATCC TIB-202; RRID: CVCL_0006 Human HeLa cells ATCC ATCC: CCL-2; RRID: CVCL_0030 Experimental models: Organisms/strains C57BL/6J mice Jackson Laboratory Cat# 000664 Mst1− /− mice Provided by Dr. Dawang Zhou Previously described in (Zhou et al., 2008) Oligonucleotides qRT-PCR primer for NLRP3 forward: 5′- CTTCTCTGATGAGGCCCAAG-3′ This paper N/A qRT-PCR primer for NLRP3 reverse: 5′- GCAGCAAACTGGAAAGGAAG-3′ This paper N/A qRT-PCR primer for IL-1β forward: 5′- GGGCCTCAAGGAAAAGAATC-3′ This paper N/A qRT-PCR primer for IL-1β reverse: 5′- TTCTGCTTGAGAGGTGCTGA-3′ This paper N/A (Continued on next page) Cell Reports 44, 116076, August 26, 2025 19 .. REAGENT or Real-time Polymerase Chain Reaction:Article Title: The Hippo pathway kinase MST1 mediates a feedback loop to maintain NLRP3 inflammasome homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Toxin B/TcdB protein, C. difficile (His) MedChemExpress Cat# HY-P79219 Poly(dA:dT)/LyoVecTM Invivogen Cat# tlrl-patc Caspase-1 (human), (recombinant) (active) Enzo Cat# ALX-201-056 MSU Invivogen Cat# tlrl-msu M-CSF Thermo Scientific Cat# PMC2044 DSS Thermo Scientific Cat# 21655 Staurosporine Solarbio Cat# S5840 ATP gamma S Kinase substrate Abcam Cat# ab138911 p-Nitrobenzyl mesylate, Alkylation reagent Abcam Cat# ab138910 Propidium Iodide MedChemExpress Cat# HY-D0815 Polybrene Sigma Cat# H9268 Digitonin Sigma Cat# D141 Doxycycline hydrochloride MedChemExpress Cat# HY-N0565A LipofectamineTM 3000 Reagent Invitrogen Cat# L3000015 Critical commercial assays Hematoxylin and Eosin Staining Kit Beyotime Cat# C0105 Human IL-6 Uncoated ELISA Kit Invitrogen Cat# 88-7066-22 Human TNF alpha Uncoated ELISA Kit Invitrogen Cat# 88-7346-22 Mouse TNF alpha Uncoated ELISA Kit Invitrogen Cat# 88-7324-22 Mouse IL-1 beta Uncoated ELISA Kit Invitrogen Cat# 88-7013A-22 Human Interleukin 1 Beta (IL-1β) ELISA Kit Shanghai Jianglai Biotechnology Cat# JL13662 LDH Cytotoxicity Assay Kit Beyotime Cat# C0017 RNAsimple Total RNA Kit TIANGEN Cat# DP419 Lentivirus Packaging Vectors Set A Beyotime Cat# L00002S 2X Universal SYBR Green Fast qPCR Mix ABclonal Cat# RK21203 ABScript III RT Master Mix ABclonal Cat# RK20429 Plasmid Midi Kit Qiagen Cat# 12143 Anti-HA Affinity Gel GNI Cat# GNI4510-HA-M Anti-Flag Affinity Gel GNI Cat# GNI4510-FG-M Protein G Plus/Protein A-Aga Merck Millipore Cat# IP10-10ML PierceTM Glutathione Agarose ThermoFisher Cat# 16100 HisPur TM Ni-NTA Resin ThermoFisher Cat# 88221 2X MultiF Seamless Assembly Mix ABclonal Cat# RK21020 Experimental models: Cell lines Human HEK293T cells ATCC ATCC CRL-11268; RRID: CVCL_1926 Human THP-1 cells ATCC ATCC TIB-202; RRID: CVCL_0006 Human HeLa cells ATCC ATCC: CCL-2; RRID: CVCL_0030 Experimental models: Organisms/strains C57BL/6J mice Jackson Laboratory Cat# 000664 Mst1− /− mice Provided by Dr. Dawang Zhou Previously described in (Zhou et al., 2008) Oligonucleotides qRT-PCR primer for NLRP3 forward: 5′- CTTCTCTGATGAGGCCCAAG-3′ This paper N/A qRT-PCR primer for NLRP3 reverse: 5′- GCAGCAAACTGGAAAGGAAG-3′ This paper N/A qRT-PCR primer for IL-1β forward: 5′- GGGCCTCAAGGAAAAGAATC-3′ This paper N/A qRT-PCR primer for IL-1β reverse: 5′- TTCTGCTTGAGAGGTGCTGA-3′ This paper N/A (Continued on next page) Cell Reports 44, 116076, August 26, 2025 19 .. REAGENT or Plasmid Preparation:Article Title: The Hippo pathway kinase MST1 mediates a feedback loop to maintain NLRP3 inflammasome homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Toxin B/TcdB protein, C. difficile (His) MedChemExpress Cat# HY-P79219 Poly(dA:dT)/LyoVecTM Invivogen Cat# tlrl-patc Caspase-1 (human), (recombinant) (active) Enzo Cat# ALX-201-056 MSU Invivogen Cat# tlrl-msu M-CSF Thermo Scientific Cat# PMC2044 DSS Thermo Scientific Cat# 21655 Staurosporine Solarbio Cat# S5840 ATP gamma S Kinase substrate Abcam Cat# ab138911 p-Nitrobenzyl mesylate, Alkylation reagent Abcam Cat# ab138910 Propidium Iodide MedChemExpress Cat# HY-D0815 Polybrene Sigma Cat# H9268 Digitonin Sigma Cat# D141 Doxycycline hydrochloride MedChemExpress Cat# HY-N0565A LipofectamineTM 3000 Reagent Invitrogen Cat# L3000015 Critical commercial assays Hematoxylin and Eosin Staining Kit Beyotime Cat# C0105 Human IL-6 Uncoated ELISA Kit Invitrogen Cat# 88-7066-22 Human TNF alpha Uncoated ELISA Kit Invitrogen Cat# 88-7346-22 Mouse TNF alpha Uncoated ELISA Kit Invitrogen Cat# 88-7324-22 Mouse IL-1 beta Uncoated ELISA Kit Invitrogen Cat# 88-7013A-22 Human Interleukin 1 Beta (IL-1β) ELISA Kit Shanghai Jianglai Biotechnology Cat# JL13662 LDH Cytotoxicity Assay Kit Beyotime Cat# C0017 RNAsimple Total RNA Kit TIANGEN Cat# DP419 Lentivirus Packaging Vectors Set A Beyotime Cat# L00002S 2X Universal SYBR Green Fast qPCR Mix ABclonal Cat# RK21203 ABScript III RT Master Mix ABclonal Cat# RK20429 Plasmid Midi Kit Qiagen Cat# 12143 Anti-HA Affinity Gel GNI Cat# GNI4510-HA-M Anti-Flag Affinity Gel GNI Cat# GNI4510-FG-M Protein G Plus/Protein A-Aga Merck Millipore Cat# IP10-10ML PierceTM Glutathione Agarose ThermoFisher Cat# 16100 HisPur TM Ni-NTA Resin ThermoFisher Cat# 88221 2X MultiF Seamless Assembly Mix ABclonal Cat# RK21020 Experimental models: Cell lines Human HEK293T cells ATCC ATCC CRL-11268; RRID: CVCL_1926 Human THP-1 cells ATCC ATCC TIB-202; RRID: CVCL_0006 Human HeLa cells ATCC ATCC: CCL-2; RRID: CVCL_0030 Experimental models: Organisms/strains C57BL/6J mice Jackson Laboratory Cat# 000664 Mst1− /− mice Provided by Dr. Dawang Zhou Previously described in (Zhou et al., 2008) Oligonucleotides qRT-PCR primer for NLRP3 forward: 5′- CTTCTCTGATGAGGCCCAAG-3′ This paper N/A qRT-PCR primer for NLRP3 reverse: 5′- GCAGCAAACTGGAAAGGAAG-3′ This paper N/A qRT-PCR primer for IL-1β forward: 5′- GGGCCTCAAGGAAAAGAATC-3′ This paper N/A qRT-PCR primer for IL-1β reverse: 5′- TTCTGCTTGAGAGGTGCTGA-3′ This paper N/A (Continued on next page) Cell Reports 44, 116076, August 26, 2025 19 .. REAGENT or Quantitative RT-PCR:Article Title: The Hippo pathway kinase MST1 mediates a feedback loop to maintain NLRP3 inflammasome homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Toxin B/TcdB protein, C. difficile (His) MedChemExpress Cat# HY-P79219 Poly(dA:dT)/LyoVecTM Invivogen Cat# tlrl-patc Caspase-1 (human), (recombinant) (active) Enzo Cat# ALX-201-056 MSU Invivogen Cat# tlrl-msu M-CSF Thermo Scientific Cat# PMC2044 DSS Thermo Scientific Cat# 21655 Staurosporine Solarbio Cat# S5840 ATP gamma S Kinase substrate Abcam Cat# ab138911 p-Nitrobenzyl mesylate, Alkylation reagent Abcam Cat# ab138910 Propidium Iodide MedChemExpress Cat# HY-D0815 Polybrene Sigma Cat# H9268 Digitonin Sigma Cat# D141 Doxycycline hydrochloride MedChemExpress Cat# HY-N0565A LipofectamineTM 3000 Reagent Invitrogen Cat# L3000015 Critical commercial assays Hematoxylin and Eosin Staining Kit Beyotime Cat# C0105 Human IL-6 Uncoated ELISA Kit Invitrogen Cat# 88-7066-22 Human TNF alpha Uncoated ELISA Kit Invitrogen Cat# 88-7346-22 Mouse TNF alpha Uncoated ELISA Kit Invitrogen Cat# 88-7324-22 Mouse IL-1 beta Uncoated ELISA Kit Invitrogen Cat# 88-7013A-22 Human Interleukin 1 Beta (IL-1β) ELISA Kit Shanghai Jianglai Biotechnology Cat# JL13662 LDH Cytotoxicity Assay Kit Beyotime Cat# C0017 RNAsimple Total RNA Kit TIANGEN Cat# DP419 Lentivirus Packaging Vectors Set A Beyotime Cat# L00002S 2X Universal SYBR Green Fast qPCR Mix ABclonal Cat# RK21203 ABScript III RT Master Mix ABclonal Cat# RK20429 Plasmid Midi Kit Qiagen Cat# 12143 Anti-HA Affinity Gel GNI Cat# GNI4510-HA-M Anti-Flag Affinity Gel GNI Cat# GNI4510-FG-M Protein G Plus/Protein A-Aga Merck Millipore Cat# IP10-10ML PierceTM Glutathione Agarose ThermoFisher Cat# 16100 HisPur TM Ni-NTA Resin ThermoFisher Cat# 88221 2X MultiF Seamless Assembly Mix ABclonal Cat# RK21020 Experimental models: Cell lines Human HEK293T cells ATCC ATCC CRL-11268; RRID: CVCL_1926 Human THP-1 cells ATCC ATCC TIB-202; RRID: CVCL_0006 Human HeLa cells ATCC ATCC: CCL-2; RRID: CVCL_0030 Experimental models: Organisms/strains C57BL/6J mice Jackson Laboratory Cat# 000664 Mst1− /− mice Provided by Dr. Dawang Zhou Previously described in (Zhou et al., 2008) Oligonucleotides qRT-PCR primer for NLRP3 forward: 5′- CTTCTCTGATGAGGCCCAAG-3′ This paper N/A qRT-PCR primer for NLRP3 reverse: 5′- GCAGCAAACTGGAAAGGAAG-3′ This paper N/A qRT-PCR primer for IL-1β forward: 5′- GGGCCTCAAGGAAAAGAATC-3′ This paper N/A qRT-PCR primer for IL-1β reverse: 5′- TTCTGCTTGAGAGGTGCTGA-3′ This paper N/A (Continued on next page) Cell Reports 44, 116076, August 26, 2025 19 .. REAGENT or |